This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: TBC1D10C, Human
PrimePCR™ Template for Probe Assay: TBC1D10C, Human
The protein encoded by this gene has an N-terminal Rab-GTPase domain and a binding site at the C-terminus for calcineurin and is an inhibitor of both the Ras signaling pathway and calcineurin a phosphatase regulated by calcium and calmodulin. Genes encoding similar proteins are located on chromsomes 16 and 22. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Feb 2012]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.