PrimePCR™ Probe Assay: INHBC, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: INHBC, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: INHBC, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054355
Assay Design:   exonic
Chromosome Location:   12:57843137-57843238question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000550133 ENST00000309668

Gene Information

This gene encodes the beta C chain of inhibin a member of the TGF-beta superfamily. This subunit forms heterodimers with beta A and beta B subunits. Inhibins and activins also members of the TGF-beta superfamily are hormones with opposing actions and are involved in hypothalamic pituitary and gonadal hormone secretion as well as growth and differentiation of various cell types. [provided by RefSeq Jul 2008]

Gene Symbol:   INHBC
Gene Name:   inhibin, beta C
Aliases:   IHBC
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000175189
Entrez:   3626
Chromosome Mapping:   12q13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997900
y-intercept 36.230000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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