PrimePCR™ Probe Assay: RSRC1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RSRC1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RSRC1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054333
Assay Design:   exonic
Chromosome Location:   3:157839966-157840066question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000480820 ENST00000494002 ENST00000295930 ENST00000471994 ENST00000464171 ENST00000312179 ENST00000475278 ENST00000476899

Gene Information

Serine- and arginine-rich (SR) proteins and SR-related proteins like RSRC1 function in spliceosome assembly and participate in multiple steps of mRNA splicing (Cazalla et al. 2005 [PubMed 15798186]).[supplied by OMIM Apr 2010]

Gene Symbol:   RSRC1
Gene Name:   arginine/serine-rich coiled-coil 1
Aliases:   MGC12197, SFRS21, SRrp53
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000174891
Entrez:   51319
Chromosome Mapping:   3q25.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996400
y-intercept 36.410000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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