PrimePCR™ Probe Assay: SRP72, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SRP72, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SRP72, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054327
Assay Design:   exonic
Chromosome Location:   4:57355644-57356596question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000342756 ENST00000510663 ENST00000505314

Gene Information

The SRP72 gene encodes the 72-kD subunit of the signal recognition particle (SRP) a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER) (summarized by Lutcke et al. 1993 [PubMed 8388879]). The complex consists of a 7S (or 7SL) RNA and 6 different proteins SRP9 (MIM 600707) SRP14 (MIM 600708) SRP19 (MIM 182175) SRP54 (MIM 604857) SRP68 (MIM 604858) and SRP72. The proteins are bound to the 7S RNA as monomers (SRP19 and SRP54) or heterodimers (SRP9/SRP14 and SRP68/SRP72). SRP9 and SRP14 constitute the Alu domain of 7S whereas the other 4 proteins belong to the S domain. SRP has at least 3 distinct functions that can be associated with the protein subunits: signal recognition translational arrest and ER membrane targeting by interaction with the docking protein.[supplied by OMIM Mar 2010]

Gene Symbol:   SRP72
Gene Name:   signal recognition particle 72kDa
Aliases:   Not Available
RefSeq:   NC_000004.11 NT_022853.15
Ensembl:   ENSG00000174780
Entrez:   6731
Chromosome Mapping:   4q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 36.010000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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