PrimePCR™ Probe Assay: SLCO4C1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SLCO4C1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SLCO4C1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054282
Assay Design:   exonic
Chromosome Location:   5:101571549-101571677question
Amplicon Length:   99
Splice Variants Targeted:   ENST00000310954

Gene Information

SLCO4C1 belongs to the organic anion transporter (OATP) family. OATPs are involved in the membrane transport of bile acids conjugated steroids thyroid hormone eicosanoids peptides and numerous drugs in many tissues (Mikkaichi et al. 2004 [PubMed 14993604]).[supplied by OMIM Mar 2008]

Gene Symbol:   SLCO4C1
Gene Name:   solute carrier organic anion transporter family, member 4C1
Aliases:   OATP-H, OATP-M1, OATP4C1, OATPX, PRO2176, SLC21A20
RefSeq:   NC_000005.9 NT_034772.6
Ensembl:   ENSG00000173930
Entrez:   353189
Chromosome Mapping:   5q21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.991800
y-intercept 35.190000
Efficiency 107

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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