PrimePCR™ Probe Assay: SCAI, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054255
Assay Design:   exonic
Chromosome Location:   9:127734017-127734115question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000336505 ENST00000373549

Gene Information

This gene encodes a regulator of cell migration. The encoded protein appears to function in the RhoA (ras homolog gene family member A)-Dia1 (diaphanous homolog 1) signal transduction pathway. Alternatively spliced transcript variants have been described. [provided by RefSeq Feb 2010]

Gene Symbol:   SCAI
Gene Name:   suppressor of cancer cell invasion
Aliases:   C9orf126, FLJ11758, FLJ36664, MGC120525, MGC120526, MGC120528, NET40
RefSeq:   NC_000009.11 NT_008470.19 NG_016620.1
Ensembl:   ENSG00000173611
Entrez:   286205
UniGene:   Hs.59504
Chromosome Mapping:   9q33.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997700
y-intercept 36.530000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download