PrimePCR™ Probe Assay: MST1, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054250
Assay Design:   exonic
Chromosome Location:   3:49724607-49724710question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000221347 ENST00000303746 ENST00000575885 ENST00000571007 ENST00000319500 ENST00000575769 ENST00000308354 ENST00000397761 ENST00000409141 ENST00000265810 ENST00000409417 ENST00000397760 ENST00000339162 ENST00000537592 ENST00000575389 ENST00000536229 ENST00000313234 ENST00000394815 ENST00000576809 ENST00000572106 ENST00000545762 ENST00000383728 ENST00000449682

Gene Information

The protein encoded by this gene contains four kringle domains and a serine protease domain similar to that found in hepatic growth factor. Despite the presence of the serine protease domain the encoded protein may not have any proteolytic activity. The receptor for this protein is RON tyrosine kinase which upon activation stimulates ciliary motility of ciliated epithelial lung cells. This protein is secreted and cleaved to form an alpha chain and a beta chain bridged by disulfide bonds. [provided by RefSeq Jan 2010]

Gene Symbol:   MST1
Gene Name:   macrophage stimulating 1 (hepatocyte growth factor-like)
Aliases:   D3F15S2, DNF15S2, HGFL, MSP, NF15S2
RefSeq:   NC_000003.11 NG_011438.1 NG_011603.1 NG_016454.1 NT_022517.18
Ensembl:   ENSG00000173531
Entrez:   4485
UniGene:   Hs.512587
Chromosome Mapping:   3p21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 36.790000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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