PrimePCR™ Probe Assay: FKBP2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: FKBP2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FKBP2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054249
Assay Design:   exonic
Chromosome Location:   11:64011348-64011570question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000379937 ENST00000546474 ENST00000309366 ENST00000449942 ENST00000394540 ENST00000427522 ENST00000467148 ENST00000356786 ENST00000359902 ENST00000301897 ENST00000354725 ENST00000246117 ENST00000592737 ENST00000337907 ENST00000377464 ENST00000476556 ENST00000400908 ENST00000376958 ENST00000542384 ENST00000586434 ENST00000401500 ENST00000270301 ENST00000511336 ENST00000264431 ENST00000514565

Gene Information

The protein encoded by this gene is a member of the immunophilin protein family which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It is thought to function as an ER chaperone and may also act as a component of membrane cytoskeletal scaffolds. Multiple alternatively spliced variants encoding the same protein have been identified. [provided by RefSeq Sep 2008]

Gene Symbol:   FKBP2
Gene Name:   FK506 binding protein 2, 13kDa
Aliases:   FKBP-13, PPIase
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000173486
Entrez:   2286
Chromosome Mapping:   11q13.1-q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 37.000000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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