PrimePCR™ Probe Assay: SNCG, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SNCG, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SNCG, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054229
Assay Design:   exonic
Chromosome Location:   10:88722820-88722947question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000289382 ENST00000340384 ENST00000296452 ENST00000427908 ENST00000264607 ENST00000355300 ENST00000254528 ENST00000452907 ENST00000324350 ENST00000526775 ENST00000585556 ENST00000306467 ENST00000515110 ENST00000348795 ENST00000372017 ENST00000367390 ENST00000394275 ENST00000418476 ENST00000261167 ENST00000537574 ENST00000559388 ENST00000394660 ENST00000302987 ENST00000394652 ENST00000558332 ENST00000368433 ENST00000315144 ENST00000368432 ENST00000292180 ENST00000368428 ENST00000569359

Gene Information

This gene encodes a member of the synuclein family of proteins which are believed to be involved in the pathogenesis of neurodegenerative diseases. Mutations in this gene have also been associated with breast tumor development. [provided by RefSeq Jan 2010]

Gene Symbol:   SNCG
Gene Name:   synuclein, gamma (breast cancer-specific protein 1)
Aliases:   BCSG1, SR
RefSeq:   NC_000010.10 NG_008783.1 NT_030059.13
Ensembl:   ENSG00000173267
Entrez:   6623
Chromosome Mapping:   10q23.2-q23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.100000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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