PrimePCR™ Probe Assay: GPR137, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054228
Assay Design:   exonic
Chromosome Location:   11:64054268-64054444question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000546139 ENST00000411458 ENST00000536667 ENST00000539833 ENST00000377702 ENST00000535675 ENST00000543383 ENST00000538032 ENST00000438980 ENST00000313074 ENST00000542190 ENST00000541952 ENST00000539851

Gene Information

Description Not Available

Gene Symbol:   GPR137
Gene Name:   G protein-coupled receptor 137
Aliases:   C11orf4, GPR137A, TM7SF1L1
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000173264
Entrez:   56834
UniGene:   Hs.523763
Chromosome Mapping:   11cen-q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.110000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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