PrimePCR™ Probe Assay: SLC2A14, Human

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054227
Assay Design:   exonic
Chromosome Location:   12:8016031-8023439question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000340749 ENST00000543909 ENST00000431042 ENST00000396589 ENST00000535295 ENST00000546234 ENST00000542782 ENST00000535344 ENST00000537557 ENST00000535266 ENST00000542916 ENST00000535383 ENST00000535587 ENST00000539234

Gene Information

Members of the glucose transporter (GLUT) family including SLC2A14 are highly conserved integral membrane proteins that transport hexoses such as glucose and fructose into all mammalian cells. GLUTs show tissue and cell-type specific expression (Wu and Freeze 2002 [PubMed 12504846]).[supplied by OMIM Mar 2008]

Gene Symbol:   SLC2A14
Gene Name:   solute carrier family 2 (facilitated glucose transporter), member 14
Aliases:   DKFZp564K1672, GLUT14, SLC2A3P3
RefSeq:   NC_000012.11 NT_009714.17
Ensembl:   ENSG00000173262
Entrez:   144195
UniGene:   Hs.655169
Chromosome Mapping:   12p13.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.060000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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