PrimePCR™ Probe Assay: ARPP21, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ARPP21, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ARPP21, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054210
Assay Design:   exonic
Chromosome Location:   3:35723226-35723348question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000450234 ENST00000428373 ENST00000421492 ENST00000444190 ENST00000449196 ENST00000187397 ENST00000452563 ENST00000438577 ENST00000419330 ENST00000427542 ENST00000474696 ENST00000412048 ENST00000396482 ENST00000432682 ENST00000432450 ENST00000413378 ENST00000417925 ENST00000396481 ENST00000441454 ENST00000436702 ENST00000438071 ENST00000458225 ENST00000337271

Gene Information

The 21-kD cAMP-regulated phosphoprotein (ARPP21) also known as regulator of calmodulin (CaM; MIM 114180) signaling (RCS) plays a central role in integration of key neurotransmitter inputs into medium spiny neurons through its regulation of CaM-dependent kinase I (CaMKI) and protein phosphatase-2B (PP2B) (Rakhilin et al. 2004 [PubMed 15499021]).[supplied by OMIM Feb 2011]

Gene Symbol:   ARPP21
Gene Name:   cAMP-regulated phosphoprotein, 21kDa
Aliases:   ARPP-21, FLJ32997, R3HDM3, RCS, TARPP
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000172995
Entrez:   10777
Chromosome Mapping:   3p22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 36.780000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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