PrimePCR™ Probe Assay: MBOAT1, Human

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054141
Assay Design:   exonic
Chromosome Location:   6:20126787-20126908question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000324607 ENST00000541730 ENST00000536798

Gene Information

MBOAT1 shares structural similarity with a superfamily of membrane-bound O-acetyltransferases that transfer organic compounds usually fatty acids (e.g. cholesterol diacylglycerol palmitoyl) onto hydroxyl groups of membrane-embedded targets (Dauwerse et al. 2007 [PubMed 17440500]).[supplied by OMIM Mar 2008]

Gene Symbol:   MBOAT1
Gene Name:   membrane bound O-acyltransferase domain containing 1
Aliases:   LPEAT1, MGC44669, OACT1, dJ434O11.1
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000172197
Entrez:   154141
UniGene:   Hs.377830
Chromosome Mapping:   6p22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997300
y-intercept 36.380000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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