PrimePCR™ Probe Assay: NME6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NME6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NME6, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054134
Assay Design:   exonic
Chromosome Location:   3:48336198-48336287question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000400848 ENST00000400847 ENST00000258456 ENST00000272462 ENST00000427178 ENST00000602922 ENST00000303395 ENST00000375405 ENST00000409050 ENST00000423058 ENST00000449719 ENST00000324288 ENST00000381835 ENST00000261800 ENST00000521544 ENST00000421967 ENST00000452211 ENST00000415644 ENST00000415053 ENST00000442597 ENST00000447314 ENST00000451657 ENST00000435684 ENST00000425930 ENST00000426689 ENST00000426723 ENST00000450160 ENST00000574949

Gene Information

Nucleoside diphosphate (NDP) kinases (EC 2.7.4.6) such as NME6 are ubiquitous enzymes that catalyze transfer of gamma-phosphates via a phosphohistidine intermediate between nucleoside and dioxynucleoside tri- and diphosphates (Mehus et al. 1999 [PubMed 10453732]).[supplied by OMIM Jul 2010]

Gene Symbol:   NME6
Gene Name:   non-metastatic cells 6, protein expressed in (nucleoside-diphosphate kinase)
Aliases:   IPIA-ALPHA, NDK 6, NM23-H6
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000172113
Entrez:   10201
Chromosome Mapping:   3p21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.100000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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