PrimePCR™ Probe Assay: QARS, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: QARS, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: QARS, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054128
Assay Design:   exonic
Chromosome Location:   3:49141795-49141904question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000306125 ENST00000414533 ENST00000452739 ENST00000417025 ENST00000420147

Gene Information

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. In metazoans 9 aminoacyl-tRNA synthetases specific for glutamine (gln) glutamic acid (glu) and 7 other amino acids are associated within a multienzyme complex. Although present in eukaryotes glutaminyl-tRNA synthetase (QARS) is absent from many prokaryotes mitochondria and chloroplasts in which Gln-tRNA(Gln) is formed by transamidation of the misacylated Glu-tRNA(Gln). Glutaminyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family. [provided by RefSeq Jul 2008]

Gene Symbol:   QARS
Gene Name:   glutaminyl-tRNA synthetase
Aliases:   GLNRS, PRO2195
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000172053
Entrez:   5859
Chromosome Mapping:   3p21.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.560000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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