PrimePCR™ Probe Assay: PIK3CD, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054088
Assay Design:   exonic
Chromosome Location:   1:9778850-9779997question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000377346 ENST00000361110 ENST00000536656 ENST00000543390

Gene Information

Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by this gene is a class I PI3K found primarily in leukocytes. Like other class I PI3Ks (p110-alpha p110-beta and p110-gamma) the encoded protein binds p85 adapter proteins and GTP-bound RAS. However unlike the other class I PI3Ks this protein phosphorylates itself not p85 protein.[provided by RefSeq Jul 2010]

Gene Symbol:   PIK3CD
Gene Name:   phosphoinositide-3-kinase, catalytic, delta polypeptide
Aliases:   P110DELTA, PI3K, p110D
RefSeq:   NC_000001.10 NG_023434.1 NT_021937.19
Ensembl:   ENSG00000171608
Entrez:   5293
UniGene:   Hs.518451
Chromosome Mapping:   1p36.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997600
y-intercept 36.640000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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