PrimePCR™ Probe Assay: ZNF584, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054085
Assay Design:   exonic
Chromosome Location:   19:58920288-58920430question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000306910 ENST00000598901 ENST00000593920 ENST00000596281 ENST00000599238 ENST00000322834

Gene Information

Description Not Available

Gene Symbol:   ZNF584
Gene Name:   zinc finger protein 584
Aliases:   FLJ39899
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000171574
Entrez:   201514
UniGene:   Hs.439551
Chromosome Mapping:   19q13.43

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 37.160000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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