PrimePCR™ Probe Assay: PLRG1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054084
Assay Design:   exonic
Chromosome Location:   4:155468877-155468988question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000499023 ENST00000302078 ENST00000504341 ENST00000393905

Gene Information

This gene encodes a core component of the cell division cycle 5-like (CDC5L) complex. The CDC5L complex is part of the spliceosome and is required for pre-mRNA splicing. The encoded protein plays a critical role in alternative splice site selection. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq Jan 2011]

Gene Symbol:   PLRG1
Gene Name:   pleiotropic regulator 1 (PRL1 homolog, Arabidopsis)
Aliases:   MGC110980, PRL1, PRP46, PRPF46
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000171566
Entrez:   5356
Chromosome Mapping:   4q31.2-q32.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997200
y-intercept 36.390000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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