PrimePCR™ Probe Assay: ZNF562, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ZNF562, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ZNF562, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054064
Assay Design:   exonic
Chromosome Location:   19:9770132-9771485question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000370353 ENST00000424629 ENST00000302851 ENST00000326044 ENST00000444611 ENST00000421525 ENST00000435550 ENST00000354661 ENST00000293648 ENST00000453372 ENST00000590155 ENST00000587392 ENST00000588653 ENST00000589542 ENST00000585350 ENST00000448622 ENST00000541032 ENST00000537617 ENST00000519811 ENST00000262585 ENST00000293201

Gene Information

Description Not Available

Gene Symbol:   ZNF562
Gene Name:   zinc finger protein 562
Aliases:   FLJ20079, MGC126160
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000171466
Entrez:   54811
Chromosome Mapping:   19p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 36.660000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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