PrimePCR™ Probe Assay: XAGE5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: XAGE5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: XAGE5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054053
Assay Design:   exonic
Chromosome Location:   X:52841661-52844117question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000182527 ENST00000375501 ENST00000351072 ENST00000425386 ENST00000375503 ENST00000209884 ENST00000395432 ENST00000338252 ENST00000395812 ENST00000341926 ENST00000511049 ENST00000309190 ENST00000511782 ENST00000504465 ENST00000510500 ENST00000437034 ENST00000508579 ENST00000395813 ENST00000359176 ENST00000325674 ENST00000593744 ENST00000601552 ENST00000600900 ENST00000594956

Gene Information

This gene is a member of the XAGE subfamily which belongs to the GAGE family. The GAGE genes are expressed in a variety of tumors and in some fetal and reproductive tissues. The protein encoded by this gene shares a sequence similarity with other GAGE/PAGE proteins. Because of the expression pattern and the sequence similarity this protein also belongs to a family of CT (cancer-testis) antigens. [provided by RefSeq Jul 2008]

Gene Symbol:   XAGE5
Gene Name:   X antigen family, member 5
Aliases:   CT12.5, GAGED5, MGC119799, MGC119800, XAGE-5
RefSeq:   NC_000023.10 NT_011630.14
Ensembl:   ENSG00000171405
Entrez:   170627
Chromosome Mapping:   Xp11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997000
y-intercept 36.190000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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