PrimePCR™ Probe Assay: ZNF212, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ZNF212, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ZNF212, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053953
Assay Design:   exonic
Chromosome Location:   7:148949870-148950739question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000335870 ENST00000481584

Gene Information

This gene belongs to the C2H2-type zinc finger gene family. The zinc finger proteins are involved in gene regulation and development and are quite conserved throughout evolution. Like this gene product a third of the zinc finger proteins containing C2H2 fingers also contain the KRAB domain which has been found to be involved in protein-protein interactions. [provided by RefSeq Jul 2008]

Gene Symbol:   ZNF212
Gene Name:   zinc finger protein 212
Aliases:   C2H2-150, MGC9707, ZNF182, ZNFC150
RefSeq:   NC_000007.13 NT_007914.15
Ensembl:   ENSG00000170260
Entrez:   7988
Chromosome Mapping:   7q36.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 36.080000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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