PrimePCR™ Probe Assay: ANKK1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ANKK1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ANKK1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053950
Assay Design:   exonic
Chromosome Location:   11:113270288-113270427question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000303941

Gene Information

The protein encoded by this gene belongs to the Ser/Thr protein kinase family and protein kinase superfamily involved in signal transduction pathways. This gene is closely linked to DRD2 gene (GeneID:1813) on chr 11 and a well studied restriction fragment length polymorphism (RFLP) designated TaqIA was originally associated with the DRD2 gene however later was determined to be located in exon 8 of ANKK1 gene (PMIDs: 18621654 15146457) where it causes a nonconservative amino acid substitution. It is not clear if this gene plays any role in neuropsychiatric disorders previously associated with Taq1A RFLP. [provided by RefSeq Sep 2009]

Gene Symbol:   ANKK1
Gene Name:   ankyrin repeat and kinase domain containing 1
Aliases:   PKK2
RefSeq:   NC_000011.9 NG_012976.1 NT_033899.8
Ensembl:   ENSG00000170209
Entrez:   255239
Chromosome Mapping:   11q23.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 37.530000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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