PrimePCR™ Probe Assay: HNRNPH1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HNRNPH1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HNRNPH1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053862
Assay Design:   exonic
Chromosome Location:   5:179050043-179050147question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000406427 ENST00000434090 ENST00000277531 ENST00000371457 ENST00000295874 ENST00000474889 ENST00000572705 ENST00000399759 ENST00000571088 ENST00000399756 ENST00000425167 ENST00000576351 ENST00000310522 ENST00000174621 ENST00000393432 ENST00000442819 ENST00000356731 ENST00000329433 ENST00000510411 ENST00000503105 ENST00000508103 ENST00000506721 ENST00000523137 ENST00000505811 ENST00000510431 ENST00000519056 ENST00000504348 ENST00000513225 ENST00000515158 ENST00000521116 ENST00000515714 ENST

Gene Information

This gene encodes a member of a subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA. These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus some may shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNA and is very similar to the family member HNRPF. This gene may be associated with hereditary lymphedema type I. Alternatively spliced transcript variants have been described [provided by RefSeq Mar 2012]

Gene Symbol:   HNRNPH1
Gene Name:   heterogeneous nuclear ribonucleoprotein H1 (H)
Aliases:   DKFZp686A15170, HNRPH, HNRPH1, hnRNPH
RefSeq:   NC_000005.9 NT_023133.13
Ensembl:   ENSG00000169045
Entrez:   3187
Chromosome Mapping:   5q35.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.920000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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