PrimePCR™ Probe Assay: BMI1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053798
Assay Design:   exonic
Chromosome Location:   10:22615471-22615906question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000602390 ENST00000376663 ENST00000442508 ENST00000416820 ENST00000443519

Gene Information

Description Not Available

Gene Symbol:   BMI1
Gene Name:   BMI1 polycomb ring finger oncogene
Aliases:   FLVI2/BMI1, MGC12685, PCGF4, RNF51
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000168283
Entrez:   648
UniGene:   Hs.380403
Chromosome Mapping:   10p11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997900
y-intercept 36.190000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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