PrimePCR™ Probe Assay: CDC42EP5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CDC42EP5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CDC42EP5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053732
Assay Design:   exonic
Chromosome Location:   19:54984263-54984370question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000535113 ENST00000284031 ENST00000539042 ENST00000426972 ENST00000542148 ENST00000535924 ENST00000379923 ENST00000541043 ENST00000375552 ENST00000338347 ENST00000440491 ENST00000281815 ENST00000369339 ENST00000369338 ENST00000301200 ENST00000436832 ENST00000401010 ENST00000339388 ENST00000442909 ENST00000446760 ENST00000369202 ENST00000369187 ENST00000442702 ENST00000417839 ENST00000303749 ENST00000522671 ENST00000577412 ENST00000581897 ENST00000583271 ENST00000579935 ENST00000580077 ENST

Gene Information

CDC42 a small Rho GTPase regulates the formation of F-actin-containing structures through its interaction with the downstream effector proteins. The protein encoded by this gene is a member of the Borg family of CDC42 effector proteins. Borg family proteins contain a CRIB (Cdc42/Rac interactive-binding) domain. They bind to and negatively regulate the function of CDC42. The specific function of this protein has not yet been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   CDC42EP5
Gene Name:   CDC42 effector protein (Rho GTPase binding) 5
Aliases:   Borg3, CEP5, MGC21945, MGC71153
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000167617
Entrez:   148170
Chromosome Mapping:   19q13.42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997200
y-intercept 38.240000
Efficiency 85

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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