PrimePCR™ Probe Assay: DUS2L, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: DUS2L, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DUS2L, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053709
Assay Design:   exonic
Chromosome Location:   16:68071921-68083416question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000571081 ENST00000570709 ENST00000575677 ENST00000576994 ENST00000566306 ENST00000565263 ENST00000358896 ENST00000568099 ENST00000567100 ENST00000432752 ENST00000569289 ENST00000564781 ENST00000562246

Gene Information

Dihydrouridine synthase catalyzes reduction of the 56-double bond of a uridine residue on the displacement loop of tRNA. The resultant modified base 56-dihydrouridine appears to increase the conformational flexibility and dynamic motion of tRNA (Kato et al. 2005 [PubMed 15994936]).[supplied by OMIM Mar 2008]

Gene Symbol:   DUS2L
Gene Name:   dihydrouridine synthase 2-like, SMM1 homolog (S. cerevisiae)
Aliases:   DUS2, FLJ20399, SMM1, URLC8
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000167264
Entrez:   54920
Chromosome Mapping:   16q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 35.810000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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