PrimePCR™ Probe Assay: TAF1D, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: TAF1D, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TAF1D, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053604
Assay Design:   exonic
Chromosome Location:   11:93471379-93471510question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000448108 ENST00000532455

Gene Information

TAF1D is a member of the SL1 complex which includes TBP (MIM 600075) and TAF1A (MIM 604903) TAF1B (MIM 604904) and TAF1C (MIM 604905) and plays a role in RNA polymerase I transcription (Wang et al. 2004 [PubMed 15520167]; Gorski et al. 2007 [PubMed 17318177]).[supplied by OMIM Jun 2009]

Gene Symbol:   TAF1D
Gene Name:   TATA box binding protein (TBP)-associated factor, RNA polymerase I, D, 41kDa
Aliases:   JOSD3, MGC5306, RAFI41, TAF(I)41
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000166012
Entrez:   79101
Chromosome Mapping:   11q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.970000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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