PrimePCR™ Probe Assay: NSD1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NSD1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NSD1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053581
Assay Design:   exonic
Chromosome Location:   5:176687164-176694653question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000358830 ENST00000394067 ENST00000316924 ENST00000394066 ENST00000417856 ENST00000421552 ENST00000439151 ENST00000347982 ENST00000508029 ENST00000503056 ENST00000504457 ENST00000505395 ENST00000515735 ENST00000361032 ENST00000354179

Gene Information

This gene encodes a protein containing a SET domain 2 LXXLL motifs 3 nuclear translocation signals (NLSs) 4 plant homeodomain (PHD) finger regions and a proline-rich region. The encoded protein enhances androgen receptor (AR) transactivation and this enhancement can be increased further in the presence of other androgen receptor associated coregulators. This protein may act as a nucleus-localized basic transcriptional factor and also as a bifunctional transcriptional regulator. Mutations of this gene have been associated with Sotos syndrome and Weaver syndrome. One version of childhood acute myeloid leukemia is the result of a cryptic translocation with the breakpoints occurring within nuclear receptor-binding Su-var enhancer of zeste and trithorax domain protein 1 on chromosome 5 and nucleoporin 98-kd on chromosome 11. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   NSD1
Gene Name:   nuclear receptor binding SET domain protein 1
Aliases:   ARA267, DKFZp666C163, FLJ10684, FLJ22263, FLJ44628, KMT3B, SOTOS, STO
RefSeq:   NC_000005.9 NG_009821.1 NT_023133.13
Ensembl:   ENSG00000165671
Entrez:   64324
Chromosome Mapping:   5q35

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.995500
y-intercept 35.640000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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