PrimePCR™ Probe Assay: MELK, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053555
Assay Design:   exonic
Chromosome Location:   9:36677278-36677416question
Amplicon Length:   109
Splice Variants Targeted:   ENST00000298048 ENST00000545008 ENST00000536860 ENST00000541717 ENST00000536329 ENST00000543751 ENST00000536987 ENST00000538311

Gene Information

Description Not Available

Gene Symbol:   MELK
Gene Name:   maternal embryonic leucine zipper kinase
Aliases:   HPK38, KIAA0175
RefSeq:   NC_000009.11 NT_008413.18
Ensembl:   ENSG00000165304
Entrez:   9833
UniGene:   Hs.184339
Chromosome Mapping:   9p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996800
y-intercept 35.830000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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