PrimePCR™ Probe Assay: SEPT8, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SEPT8, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SEPT8, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053478
Assay Design:   exonic
Chromosome Location:   5:132099998-132100088question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000359600 ENST00000378719 ENST00000378721 ENST00000296873 ENST00000378706 ENST00000378699 ENST00000378701 ENST00000458488 ENST00000453480 ENST00000414594 ENST00000371493 ENST00000448933 ENST00000322122 ENST00000313164

Gene Information

SEPT8 is a member of the highly conserved septin family. Septins are 40- to 60-kD GTPases that assemble as filamentous scaffolds. They are involved in the organization of submembranous structures in neuronal polarity and in vesicle trafficking (Blaser et al. 2003 [PubMed 12909369]).[supplied by OMIM Mar 2008]

Gene Symbol:   SEPT8
Gene Name:   septin 8
Aliases:   KIAA0202, SEP2
RefSeq:   NC_000005.9 NT_034772.6
Ensembl:   ENSG00000164402
Entrez:   23176
Chromosome Mapping:   5q31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.150000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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