PrimePCR™ Probe Assay: OTUD4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053447
Assay Design:   exonic
Chromosome Location:   4:146077142-146080787question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000454497 ENST00000447906 ENST00000514973 ENST00000509620 ENST00000504501 ENST00000296579

Gene Information

Alternatively spliced transcript variants have been found for this gene. The smaller protein isoform encoded by the shorter transcript variant is found only in HIV-1 infected cells. [provided by RefSeq Jul 2010]

Gene Symbol:   OTUD4
Gene Name:   OTU domain containing 4
Aliases:   DKFZp434I0721, DUBA6, HIN1, HSHIN1, KIAA1046
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000164164
Entrez:   54726
UniGene:   Hs.270851
Chromosome Mapping:   4q31.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 36.490000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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