PrimePCR™ Probe Assay: HS3ST6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: HS3ST6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HS3ST6, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053223
Assay Design:   exonic
Chromosome Location:   16:1962023-1962135question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000331351 ENST00000416258 ENST00000454677 ENST00000293937 ENST00000443547 ENST00000299732 ENST00000416617 ENST00000546692 ENST00000305921 ENST00000231721 ENST00000284110 ENST00000578576 ENST00000360954 ENST00000367329 ENST00000367330 ENST00000261658 ENST00000563971 ENST00000368102 ENST00000396667

Gene Information

Description Not Available

Gene Symbol:   HS3ST6
Gene Name:   heparan sulfate (glucosamine) 3-O-sulfotransferase 6
Aliases:   HS3ST5
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000162040
Entrez:   64711
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 35.480000
Efficiency 106

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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