PrimePCR™ Probe Assay: SRSF2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SRSF2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SRSF2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053199
Assay Design:   exonic
Chromosome Location:   17:74732910-74733040question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000340415 ENST00000402449 ENST00000528438 ENST00000392614 ENST00000392613 ENST00000392612 ENST00000392610 ENST00000379499 ENST00000416488 ENST00000540096 ENST00000521891 ENST00000518282 ENST00000455469 ENST00000050961 ENST00000277120 ENST00000323115 ENST00000376214 ENST00000376213 ENST00000274979 ENST00000402430 ENST00000327726 ENST00000591864 ENST00000588460 ENST00000586622 ENST00000358156 ENST00000583836 ENST00000508921 ENST00000359995 ENST00000392485

Gene Information

The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants encoding the same protein and one non-coding transcript variant have been found for this gene. In addition a pseudogene of this gene has been found on chromosome 11. [provided by RefSeq Sep 2010]

Gene Symbol:   SRSF2
Gene Name:   serine/arginine-rich splicing factor 2
Aliases:   PR264, SC-35, SC35, SFRS2, SFRS2A, SRp30b
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000161547
Entrez:   6427
Chromosome Mapping:   17q25.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996900
y-intercept 36.030000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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