PrimePCR™ Probe Assay: RGL4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
Your Price:   Log In
Quantity:  
 

PrimePCR™ PreAmp for Probe Assay: RGL4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RGL4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053120
Assay Design:   exonic
Chromosome Location:   22:24041076-24041185question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000300648 ENST00000262518 ENST00000546530 ENST00000295101 ENST00000544049 ENST00000553525 ENST00000555571 ENST00000553530 ENST00000556437 ENST00000355512 ENST00000404301 ENST00000406236 ENST00000407322 ENST00000434263 ENST00000402788 ENST00000343854 ENST00000302956 ENST00000380913 ENST00000418909 ENST00000588649 ENST00000215591 ENST00000342740 ENST00000334420 ENST00000265626 ENST00000218104 ENST00000527990 ENST00000254579 ENST00000358077 ENST00000472284 ENST00000408954 ENST00000491893 ENST

Gene Information

Description Not Available

Gene Symbol:   RGL4
Gene Name:   ral guanine nucleotide dissociation stimulator-like 4
Aliases:   MGC119678, MGC119680, Rgr
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000159496
Entrez:   266747
Chromosome Mapping:   22q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.840000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download