PrimePCR™ Probe Assay: NLRP14, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NLRP14, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NLRP14, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053060
Assay Design:   exonic
Chromosome Location:   11:7065210-7068034question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000299481

Gene Information

The protein encoded by this gene belongs to the NALP protein family. Members of the NALP protein family typically contain a NACHT domain a NACHT-associated domain (NAD) a C-terminal leucine-rich repeat (LRR) region and an N-terminal pyrin domain (PYD). This protein may play a regulatory role in the innate immune system as similar family members belong to the signal-induced multiprotein complex the inflammasome that activates the pro-inflammatory caspases caspase-1 and caspase-5. [provided by RefSeq Jul 2008]

Gene Symbol:   NLRP14
Gene Name:   NLR family, pyrin domain containing 14
Aliases:   CLR11.2, GC-LRR, NALP14, NOD5, PAN8
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000158077
Entrez:   338323
Chromosome Mapping:   11p15.4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.000000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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