PrimePCR™ Probe Assay: MAGEA8, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MAGEA8, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MAGEA8, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052965
Assay Design:   exonic
Chromosome Location:   X:149013706-149013935question
Amplicon Length:   200
Splice Variants Targeted:   ENST00000243314 ENST00000298974 ENST00000519822 ENST00000286482 ENST00000535454 ENST00000542674 ENST00000595065 ENST00000598469 ENST00000595910 ENST00000600776 ENST00000597872 ENST00000596200

Gene Information

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders such as dyskeratosis congenita. Multiple alternatively spliced variants encoding the same protein have been identified. [provided by RefSeq Oct 2009]

Gene Symbol:   MAGEA8
Gene Name:   melanoma antigen family A, 8
Aliases:   CT1.8, MAGE8, MGC2182
RefSeq:   NC_000023.10 NT_011681.16 NG_015868.1
Ensembl:   ENSG00000156009
Entrez:   4107
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.320000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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