PrimePCR™ Probe Assay: MMAA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052787
Assay Design:   exonic
Chromosome Location:   4:146560391-146560527question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000281317 ENST00000541599

Gene Information

The protein encoded by this gene is involved in the translocation of cobalamin into the mitochondrion where it is used in the final steps of adenosylcobalamin synthesis. Adenosylcobalamin is a coenzyme required for the activity of methylmalonyl-CoA mutase. Defects in this gene are a cause of methylmalonic aciduria. [provided by RefSeq Jul 2008]

Gene Symbol:   MMAA
Gene Name:   methylmalonic aciduria (cobalamin deficiency) cblA type
Aliases:   MGC120010, MGC120011, MGC120012, MGC120013, cblA
RefSeq:   NC_000004.11 NG_007536.1 NT_016354.19
Ensembl:   ENSG00000151611
Entrez:   166785
Chromosome Mapping:   4q31.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.440000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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