PrimePCR™ Probe Assay: LPHN3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: LPHN3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LPHN3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052741
Assay Design:   exonic
Chromosome Location:   4:62936297-62936430question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000264033 ENST00000512091 ENST00000514591 ENST00000509896 ENST00000511324 ENST00000506700 ENST00000507164 ENST00000508693 ENST00000507625 ENST00000514157 ENST00000504896 ENST00000508946 ENST00000506720 ENST00000506746 ENST00000514996 ENST00000502815 ENST00000545650 ENST00000574536 ENST00000571630 ENST00000573720 ENST00000576469 ENST00000572079 ENST00000576028 ENST00000574839 ENST00000573960 ENST00000572342 ENST00000576325 ENST00000574919 ENST00000574812 ENST00000577151 ENST00000573738 ENST

Gene Information

This gene encodes a member of the latrophilin subfamily of G-protein coupled receptors (GPCR). Latrophilins may function in both cell adhesion and signal transduction. In experiments with non-human species endogenous proteolytic cleavage within a cysteine-rich GPS (G-protein-coupled-receptor proteolysis site) domain resulted in two subunits (a large extracellular N-terminal cell adhesion subunit and a subunit with substantial similarity to the secretin/calcitonin family of GPCRs) being non-covalently bound at the cell membrane. [provided by RefSeq Jul 2008]

Gene Symbol:   LPHN3
Gene Name:   latrophilin 3
Aliases:   CIRL3, LEC3
RefSeq:   NC_000004.11 NT_022778.16 NW_003315916.1
Ensembl:   ENSG00000150471
Entrez:   23284
Chromosome Mapping:   4q13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996900
y-intercept 35.550000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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