PrimePCR™ Probe Assay: CPSF7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: CPSF7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CPSF7, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052717
Assay Design:   exonic
Chromosome Location:   11:61189070-61197240question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000340437 ENST00000394888 ENST00000439958 ENST00000448745 ENST00000477890 ENST00000539952 ENST00000544585 ENST00000413232 ENST00000541963 ENST00000450000 ENST00000449811 ENST00000413184

Gene Information

Description Not Available

Gene Symbol:   CPSF7
Gene Name:   cleavage and polyadenylation specific factor 7, 59kDa
Aliases:   CFIm59, FLJ12529, FLJ39024, MGC9315
RefSeq:   NC_000011.9 NG_023393.1 NT_167190.1
Ensembl:   ENSG00000149532
Entrez:   79869
Chromosome Mapping:   11q12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.620000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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