PrimePCR™ Probe Assay: TRIM50, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: TRIM50, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TRIM50, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052600
Assay Design:   exonic
Chromosome Location:   7:72727361-72727480question
Amplicon Length:   90
Splice Variants Targeted:   ENST00000473149 ENST00000477737 ENST00000263672 ENST00000312397 ENST00000503335 ENST00000274473 ENST00000400454 ENST00000404019 ENST00000340124 ENST00000440783 ENST00000504548 ENST00000321385 ENST00000321613 ENST00000454243 ENST00000303143 ENST00000397799 ENST00000267328 ENST00000374127 ENST00000508005 ENST00000447839 ENST00000515869 ENST00000520200 ENST00000261800 ENST00000370606 ENST00000369146 ENST00000227756 ENST00000333149 ENST00000453152 ENST00000358595 ENST00000354396 ENST00000525322 ENST

Gene Information

Description Not Available

Gene Symbol:   TRIM50
Gene Name:   tripartite motif containing 50
Aliases:   FLJ32804, MGC138357, MGC138359, TRIM50A
RefSeq:   NC_000007.13 NG_023242.1 NT_007933.15
Ensembl:   ENSG00000146755
Entrez:   135892
Chromosome Mapping:   7q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.750000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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