PrimePCR™ Probe Assay: ADAMTS16, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ADAMTS16, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ADAMTS16, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052535
Assay Design:   exonic
Chromosome Location:   5:5182166-5182311question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000511368 ENST00000274181

Gene Information

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. ADAMTS family members share several distinct protein modules including a propeptide region a metalloproteinase domain a disintegrin-like domain and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs and some have unique C-terminal domains. The protein encoded by this gene has high sequence similarity to the protein encoded by ADAMTS18 another family member. [provided by RefSeq Jul 2008]

Gene Symbol:   ADAMTS16
Gene Name:   ADAM metallopeptidase with thrombospondin type 1 motif, 16
Aliases:   ADAMTS16s, FLJ16731
RefSeq:   NC_000005.9 NT_006576.16
Ensembl:   ENSG00000145536
Entrez:   170690
Chromosome Mapping:   5p15

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.240000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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