PrimePCR™ Probe Assay: ALDH1L1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ALDH1L1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ALDH1L1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052498
Assay Design:   exonic
Chromosome Location:   3:125879701-125879838question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000273450 ENST00000472186 ENST00000452905 ENST00000393434 ENST00000490367 ENST00000460368 ENST00000488356 ENST00000509952 ENST00000393431 ENST00000455064

Gene Information

The protein encoded by this gene catalyzes the conversion of 10-formyltetrahydrofolate NADP and water to tetrahydrofolate NADPH and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family and is responsible for formate oxidation in vivo. Deficiencies in this gene can result in an accumulation of formate and subsequent methanol poisoning. [provided by RefSeq Jul 2008]

Gene Symbol:   ALDH1L1
Gene Name:   aldehyde dehydrogenase 1 family, member L1
Aliases:   10-fTHF, DKFZp781N0997, FDH, FTHFD
RefSeq:   NC_000003.11 NG_012260.1 NT_005612.16
Ensembl:   ENSG00000144908
Entrez:   10840
Chromosome Mapping:   3q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.060000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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