This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: PKP4, Human
PrimePCR™ Template for Probe Assay: PKP4, Human
Armadillo-like proteins are characterized by a series of armadillo repeats first defined in the Drosophila 'armadillo' gene product that are typically 42 to 45 amino acids in length. These proteins can be divided into subfamilies based on their number of repeats their overall sequence similarity and the dispersion of the repeats throughout their sequences. Members of the p120(ctn)/plakophilin subfamily of Armadillo-like proteins including CTNND1 CTNND2 PKP1 PKP2 PKP4 and ARVCF. PKP4 may be a component of desmosomal plaque and other adhesion plaques and is thought to be involved in regulating junctional plaque organization and cadherin function. Multiple transcript variants have been found for this gene but the full-length nature of only two of them have been described so far. These two variants encode distinct isoforms. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.