PrimePCR™ Probe Assay: MEIS1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052433
Assay Design:   exonic
Chromosome Location:   2:66670035-66670170question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000560281 ENST00000272369 ENST00000398506 ENST00000495021 ENST00000409622 ENST00000437869 ENST00000407092 ENST00000444274

Gene Information

Homeobox genes of which the most well-characterized category is represented by the HOX genes play a crucial role in normal development. In addition several homeoproteins are involved in neoplasia. This gene encodes a homeobox protein belonging to the TALE ('three amino acid loop extension') family of homeodomain-containing proteins. [provided by RefSeq Jul 2008]

Gene Symbol:   MEIS1
Gene Name:   Meis homeobox 1
Aliases:   MGC43380
RefSeq:   NC_000002.11 NG_011467.1 NT_022184.15
Ensembl:   ENSG00000143995
Entrez:   4211
UniGene:   Hs.526754
Chromosome Mapping:   2p14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.790000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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