PrimePCR™ Probe Assay: SF3B4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SF3B4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SF3B4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052382
Assay Design:   exonic
Chromosome Location:   1:149898807-149899184question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000585708 ENST00000586184 ENST00000588077 ENST00000587995 ENST00000382722 ENST00000215917 ENST00000464792 ENST00000271628 ENST00000457312 ENST00000582851 ENST00000582075

Gene Information

This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B C and E spliceosomal complexes. In addition to RNA-binding activity this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs) consistent with the observation that it binds directly to pre-mRNA. [provided by RefSeq Jul 2008]

Gene Symbol:   SF3B4
Gene Name:   splicing factor 3b, subunit 4, 49kDa
Aliases:   Hsh49, MGC10828, SAP49, SF3b49
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000143368
Entrez:   10262
Chromosome Mapping:   1q21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.080000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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