PrimePCR™ Probe Assay: NR1I3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NR1I3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NR1I3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052369
Assay Design:   exonic
Chromosome Location:   1:161200614-161200711question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000512372 ENST00000367983 ENST00000437437 ENST00000442691 ENST00000412844 ENST00000428574 ENST00000505005 ENST00000508740 ENST00000367982 ENST00000508387 ENST00000504010 ENST00000511676 ENST00000502985 ENST00000367981 ENST00000515621 ENST00000511944 ENST00000511748 ENST00000367984 ENST00000367985 ENST00000367979 ENST00000506209 ENST00000367980

Gene Information

This gene encodes a member of the nuclear receptor superfamily and is a key regulator of xenobiotic and endobiotic metabolism. The protein binds to DNA as a monomer or a heterodimer with the retinoid X receptor and regulates the transcription of target genes involved in drug metabolism and bilirubin clearance such as cytochrome P450 family members. Unlike most nuclear receptors this transcriptional regulator is constitutively active in the absence of ligand but is regulated by both agonists and inverse agonists. Ligand binding results in translocation of this protein to the nucleus where it activates or represses target gene transcription. These ligands include bilirubin a variety of foreign compounds steroid hormones and prescription drugs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   NR1I3
Gene Name:   nuclear receptor subfamily 1, group I, member 3
Aliases:   CAR, CAR1, MB67, MGC150433, MGC97144, MGC97209
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000143257
Entrez:   9970
Chromosome Mapping:   1q23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.840000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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