PrimePCR™ Probe Assay: ARMC5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ARMC5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ARMC5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052238
Assay Design:   exonic
Chromosome Location:   16:31477539-31477629question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000383020 ENST00000382707 ENST00000439108 ENST00000382659 ENST00000382673 ENST00000382680 ENST00000303766 ENST00000454978 ENST00000414629 ENST00000445779 ENST00000572428 ENST00000299866 ENST00000572102 ENST00000481401 ENST00000408912 ENST00000457010 ENST00000563544 ENST00000564900 ENST00000538189 ENST00000268314 ENST00000412665 ENST00000217233 ENST00000449710 ENST00000422053 ENST00000267082 ENST00000422257 ENST00000338737

Gene Information

Description Not Available

Gene Symbol:   ARMC5
Gene Name:   armadillo repeat containing 5
Aliases:   FLJ00019, FLJ13063
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000140691
Entrez:   79798
Chromosome Mapping:   16p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.610000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
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