PrimePCR™ Probe Assay: MAN2C1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
Your Price:   Log In
Quantity:  
 

PrimePCR™ PreAmp for Probe Assay: MAN2C1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MAN2C1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052223
Assay Design:   exonic
Chromosome Location:   15:75658928-75659938question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000481290 ENST00000531214 ENST00000320230 ENST00000388726 ENST00000424778 ENST00000392650 ENST00000602922 ENST00000217320 ENST00000300527 ENST00000409416 ENST00000397763 ENST00000357838 ENST00000310645 ENST00000407373 ENST00000332585 ENST00000446658 ENST00000401475 ENST00000431368 ENST00000452656 ENST00000403222 ENST00000382310 ENST00000437268 ENST00000535268 ENST00000569482 ENST00000565683 ENST00000563622 ENST00000267978 ENST00000568374 ENST00000566256 ENST00000561615 ENST00000259235 ENST

Gene Information

Description Not Available

Gene Symbol:   MAN2C1
Gene Name:   mannosidase, alpha, class 2C, member 1
Aliases:   DKFZp686E23167, MAN6A8, MANA, MANA1, MGC87979
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000140400
Entrez:   4123
Chromosome Mapping:   15q11-q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.730000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download