PrimePCR™ Probe Assay: HDC, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: HDC, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HDC, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052215
Assay Design:   exonic
Chromosome Location:   15:50550642-50555449question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000371477 ENST00000383785 ENST00000435459 ENST00000383781 ENST00000383786 ENST00000383787 ENST00000383788 ENST00000545661 ENST00000334583 ENST00000393160 ENST00000575270 ENST00000513328 ENST00000503455 ENST00000355842 ENST00000398129 ENST00000264758 ENST00000446856 ENST00000398125 ENST00000309166 ENST00000534759 ENST00000267845 ENST00000543581 ENST00000559683 ENST00000559513 ENST00000377263 ENST00000537447 ENST00000257901 ENST00000544265 ENST00000373542 ENST00000373539 ENST00000339490 ENST

Gene Information

This gene encodes a member of the group II decarboxylase family and forms a homodimer that converts L-histidine to histamine in a pyridoxal phosphate dependent manner. Histamine regulates several physiologic processes including neurotransmission gastric acid secretioninflamation and smooth muscle tone.[provided by RefSeq Aug 2010]

Gene Symbol:   HDC
Gene Name:   histidine decarboxylase
Aliases:   MGC163399
RefSeq:   NC_000015.9 NG_027487.1 NT_010194.17
Ensembl:   ENSG00000140287
Entrez:   3067
Chromosome Mapping:   15q21-q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 36.890000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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