PrimePCR™ Probe Assay: SEMA6D, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SEMA6D, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SEMA6D, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052054
Assay Design:   exonic
Chromosome Location:   15:48053405-48053858question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000558014 ENST00000561133 ENST00000536845 ENST00000558816 ENST00000389425 ENST00000316364 ENST00000354744 ENST00000358066 ENST00000389428 ENST00000559196 ENST00000537942 ENST00000389433 ENST00000389432 ENST00000355997

Gene Information

Semaphorins are a large family including both secreted and membrane associated proteins many of which have been implicated as inhibitors or chemorepellents in axon pathfinding fasciculation and branching and target selection. All semaphorins possess a semaphorin (Sema) domain and a PSI domain (found in plexins semaphorins and integrins) in the N-terminal extracellular portion. Additional sequence motifs C-terminal to the semaphorin domain allow classification into distinct subfamilies. Results demonstrate that transmembrane semaphorins like the secreted ones can act as repulsive axon guidance cues. This gene encodes a class 6 vertebrate transmembrane semaphorin that demonstrates alternative splicing. Several transcript variants have been identified and expression of the distinct encoded isoforms is thought to be regulated in a tissue- and development-dependent manner. [provided by RefSeq Nov 2010]

Gene Symbol:   SEMA6D
Gene Name:   sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D
Aliases:   FLJ11598, KIAA1479
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000137872
Entrez:   80031
Chromosome Mapping:   15q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.640000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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